Email updates

Keep up to date with the latest news and content from Infectious Agents and Cancer and BioMed Central.

This article is part of the supplement: Proceedings of the 12th International Conference on Malignancies in AIDS and Other Acquired Immunodeficiencies (ICMAOI)

Open Access Meeting abstracts

Detection and quantitation of HPV in anogenital and oral tissues and fluids of HIV-positive individuals by real-time PCR

William T Seaman1*, Elizabeth Andrews24, Marion Couch1, Erna Milu Kojic5, Susan Cu-Uvin5, Allison M Deal4, Byrd Quinlavin5, Julia Seay5 and Jennifer Webster-Cyriaque123

Author Affiliations

1 Lineberger Cancer Center, University of North Carolina, Chapel Hill, NC, USA

2 Division of Infectious Disease, University of North Carolina, Chapel Hill, NC, USA

3 Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, NC, USA

4 Department of Dental Ecology, University of North Carolina School of Dentistry, Chapel Hill, NC, USA

5 Division of Biology and Medicine, Brown University, Providence, RI, USA

For all author emails, please log on.

Infectious Agents and Cancer 2010, 5(Suppl 1):A17  doi:10.1186/1750-9378-5-S1-A17


The electronic version of this article is the complete one and can be found online at: http://www.infectagentscancer.com/content/5/S1/A17


Published:11 October 2010

© 2010 Seaman et al; licensee BioMed Central Ltd.

Meeting abstracts

Human papillomaviruses (HPV) remain a serious world health problem due to their association with anogenital and oral cancers and warts. While over 100 HPV types have been identified, only a subset is associated with malignancy. HPV16 and 18 are the most common oncogenic types, while HPV6 and 11 are the most common types responsible for anogenital warts. These four types cause up to 90% of HPV-associated disease. While other quantitative PCR (qPCR) assays can be used to detect oncogenic HPV, there is no single tube assay that distinguishes the most frequent oncogenic types and the most common types found in warts. A qPCR assay was developed that allowed for detection and quantitation of these 4 HPV types. Type-specific primer pairs and TaqMan probes allowed single tube multiplex reactions to be performed. Each HPV type was detected over a range from 2 Η 101 to 2 Η 106 copies/reaction, providing a reliable method of quantitating type-specific HPV. A Sybr Green-based qPCR assay was developed that utilizes degenerate primers targeting the E1 region of all HPVs. These assays were run in parallel with PCR/sequence gold standard on 76 oral cancers from HIV-negative individuals. Cervical and oral washes were collected from 25 HIV-positive women and 90 HIV-positive men, respectively, being screened for anogenital neoplasia. Samples were analyzed using the newly developed assays. Of the 115 samples, 16% were HPV positive. Cervical washes contained HPV types 44, 67, 35, and 68 and oral specimens contained HPV types 16, 11, 32, 6, 55, 73, and 70. These results indicate that these assays can be used to detect and quantitate HPV in clinical samples obtained by noninvasive measures.

Acknowledgements

This work was supported in part by NIDCR OHARA 1 U01 AI068636-01.

This article has been published as part of Infectious Agents and Cancer Volume 5 Supplement 1, 2010: Proceedings of the 12th International Conference on Malignancies in AIDS and Other Acquired Immunodeficiencies (ICMAOI).The full contents of the supplement are available online at http://www.biomedcentral.com/1750-9378/5?issue=S1.