Role of chronic E. coli infection in the process of bladder cancer- an experimental study
1 Biochemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
2 Biochemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt
3 Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt
4 Biochemistry Department, Faculty of Pharmacy, Modern Sciences and Arts University, Cairo, Egypt
Infectious Agents and Cancer 2012, 7:19 doi:10.1186/1750-9378-7-19Published: 8 August 2012
Bladder cancer is a common malignancy in Egypt. A history of urinary tract infection can be considered as a risk factor for bladder cancer. Escherichia coli (E. coli) infection is responsible for 70% of urinary tract infection. This study aimed to evaluate the role of chronic E. coli infection during bladder carcinogenesis. In order to achieve this aim, we investigated the histopathological changes in bladder tissue and measured the level of nuclear factor kappa p65 (NF-κBp65), Bcl-2 and interleukin 6 (IL-6) in four groups each consisting of 25 male albino rats except of control group consisting of 20 rats. The first group was normal control group, the second group was infected with E. coli, the third group was administered nitrosamine precursor, and the forth group was infected with E. coli and administered nitrosamine precursor.
The histopathological examination revealed that E. coli infected group was able alone to produce some histopathological changes in bladder tissue and that nitrosamine precursor plus E. coli group showed highest incidences of urinary bladder lesions than the nitrosamine precursor group. NF-κBp65, Bcl-2 and IL-6 levels were significantly higher in nitrosamine precursor plus E. coli group than the other groups.
These findings suggested that urinary bladder infection by E. coli may play a major additive and synergistic role during bladder carcinogenesis.